Description
This Rat GDNF Family Receptor Alpha-2 (GFRA2) ELISA Kit is intended for quantitative detection of rat GFRA2 in cell culture supernates, serum and plasma (heparin, EDTA, citrate). Strip well format. Reagents for up to 96 tests.
This rat GFRA2 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for GFRA2 has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: S22-S443) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for GFRA2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat GFRA2 amount of sample captured in plate.
The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: GDNF family receptor alpha-2 (GFRalpha2), also known as the neurturin receptor, is a protein that in humans is encoded by the GFRA2 gene. It is mapped to 15p11. The GFRA2 protein is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor. It is part of the GDNF receptor family. Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. They both bind the GFRA2 receptor. The receptor mediates activation of the RET tyrosine kinase receptor. This encoded protein acts preferentially as a receptor for NTN compared to its other family member, GDNF family receptor alpha 1. This gene is a candidate gene for RET-associated diseases. GFRA2 (gene) has been shown to interact with GDNF